Identification and tissue localization of intermediate filament protein in Angiostrongylus cantonensis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1141-1144, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-337311
ABSTRACT
<p><b>OBJECTIVE</b>To identify the type of the intermediate filament (IF) protein of Angiostrongylus cantonensis and analyze its tissue localization.</p><p><b>METHODS</b>Recombinant pET-IF of antigen IF was expressed in E.coli with IPTG induction, and the expression products were purified by His.Bind column and identified for determining the type of the IF protein by Western blotting. Anti-IF antibody was prepared by multi-spot subcutaneous injection into mouse and used to detect the tissue slices of A. cantonensis by immunohistochemical analysis.</p><p><b>RESULTS</b>The antigen IF were correctly expressed and purified, and identified as a keratin located in the intestine wall and cytoplusma.</p><p><b>CONCLUSION</b>The antigen IF is distributed in the intestine wall of A. cantonensis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Nucleus
/
Classification
/
Angiostrongylus cantonensis
/
Protein Transport
/
Cell Biology
/
Electrophoresis, Polyacrylamide Gel
/
Genetics
/
Intermediate Filament Proteins
/
Metabolism
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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