Cloning and functional study of a novel aromatic-ring-hydroxylating dioxygenase gene / 南方医科大学学报
Journal of Southern Medical University
;
(12): 717-719, 2007.
Article
in English
| WPRIM
| ID: wpr-337357
ABSTRACT
The aromatic-ring-hydroxylating dioxygenase is a key enzyme that initiates the biodegradation of polycyclic aromatic hydrocarbons in bacteria. In the present study, a novel dioxygenase sequence was cloned from Terrabacter sp. FLO using a genome walking method. The dioxygenase was cloned into pET17 and actively expressed in E.coli BL21 (DE3) in co-expression with electron transfer chain proteins. The recombinant dioxygenase was found to transform phenanthrene, fluorene, pyrene and fluoranthene into the cis-dihydrodiol metabolites.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenanthrenes
/
Polycyclic Aromatic Hydrocarbons
/
Pyrenes
/
Bacterial Proteins
/
Biodegradation, Environmental
/
Recombinant Proteins
/
Actinomycetales
/
Molecular Sequence Data
/
Cloning, Molecular
/
Dioxygenases
Language:
English
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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