Analysis of class I integrons in metalloenzyme-producing Pseudomonas aeruginosa / 南方医科大学学报
Journal of Southern Medical University
;
(12): 792-794, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-337383
ABSTRACT
<p><b>OBJECTIVE</b>To investigate class I integrons and integrated gene cassettes in metalloenzyme-producing Pseudomonas aeruginosa.</p><p><b>METHODS</b>A total of 68 isolated clinical strains of Pseudomonas aeruginosa were subjected to PCR analysis with primers specific for bla(IMP-1) and bla(VIM). The positive strains then underwent examination for class I integrons and integrated gene cassettes with PCR with primers specific to class I integrase ((IntI)1) and integrated gene cassettes, followed by sequence analysis for some of the positive strains.</p><p><b>RESULTS</b>Only 1 isolated strain showed positive results for both bla(IMP-1) and bla IntI1 detection. Fifty-five strains were positive for bla(VIM), including 26 positive for bla (IntI)1. Of the 26 bla (IntI)1-positive strains, only 18 contained integrated gene cassettes, which were classified into 5 types according to agarose gel electrophoresis.</p><p><b>CONCLUSION</b>It is the first time to identify IMP-1-producing Pseudomonas aeruginosa carring bla(Int)1 in West China. The class I integrons were widespread in these Pseudomonas aeruginosa and 69.2% of them carry the gene cassettes. These findings provide useful insights into the clinical spread of these drug-resistant genes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pseudomonas aeruginosa
/
Pseudomonas Infections
/
Species Specificity
/
Bacterial Proteins
/
Beta-Lactamases
/
DNA, Bacterial
/
Polymerase Chain Reaction
/
Drug Resistance, Bacterial
/
Integrons
/
Electrophoresis, Agar Gel
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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