One-step fermentation for producing simvastatin via RNAi silencing of lovF gene in Aspergillus terreus / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 478-486, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-337449
ABSTRACT
Simvastatin is one of the important prescription drugs for hypercholesterolemia. Monacolin J is a key intermediate during simvastatin synthesis, and also an intermediate of lovastatin biosynthesis. In this work, we construct a monacolin J producing strain via RNA interference to achieve one-step fermentation to obtain simvastatin. The lovF gene silencing plasmid pMHJ137 was constructed and transformed into Aspergillus terreus by Agrobacterium tumefaciens mediated transformation method. Precursor DMB-S-MMP was supplied during the fermentation to screen positive strains of transformants; which also further confirmed the simvastatin producing capability of MJ1-24 by one-step fermentation. Strain MJ1-24 produced monacolin J rather than lovastatin, and the feeding of DMB-S-MMP resulted in the generation of simvastatin. This study suggested that RNAi can efficiently silence the lovF gene of A. terreus and promote the simvastatin production by one-step fermentation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aspergillus
/
Industrial Microbiology
/
Chemistry
/
Simvastatin
/
RNA Interference
/
Fermentation
/
Genetics
/
3-Mercaptopropionic Acid
/
Naphthalenes
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2016
Type:
Article
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