Automatically feeding strategy for 1,3-propanediol fermentation of Klebsiella pneumoniae LDH526 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1520-1527, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-337469
ABSTRACT
1,3-propanediol is an important chemical used as building block for the synthesis of highly promising polyesters such as polytrimethylene terephthalate. A genetically modified Klebsiella pneumoniae LDH526 can use glycerol as sole carbon source and produce 1,3-propanediol with the titer above 90 g/L. A key factor affecting the production of 1,3-propanediol by the mutant K. pneumoniae is the accurate control of the feeding of glycerol. To generate a robust and reproducible fermentation process of 1,3-propanediol, we designed and optimized an automatically feeding strategy of glycerol based on fermentation kinetics. By coupling the substrate feeding rate with easily observed variables -pH and fermentation time, we have achieved self-starting glycerol feeding and dynamic control of the glycerol concentration during the fermentation process. This automated system allowed us to generate a reproducible, consistent and operator-independent process from lab-scale to production scale. The final concentration of 1,3-propanediol was above 95 g/L after 72 h.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Propylene Glycols
/
Industrial Microbiology
/
Propylene Glycol
/
Culture Media
/
Fermentation
/
Glycerol
/
Klebsiella pneumoniae
/
Metabolism
/
Methods
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2015
Type:
Article
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