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Breeding of high-producing LI-F lipopeptide Paenibacillus polymyxa by protoplast fusion and differential expression analysis of fusion strains / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1401-1407, 2015.
Article in Chinese | WPRIM | ID: wpr-337480
ABSTRACT
Auxotrophic strains of N1-37 (Phe-) and N2-27 (His-), screened from mutations of Paenibacillus polymyxa JSa-9 previously, were used as the parent strains to screen high-producing LI-F antibacterial lipopeptide fusion strain through protoplast fusion with polyethylene glycol as a promote agent. Fusion strain F5-15 was obtained. Then the product of LI-F antibacterial lipopeptide was quantified by HPLC, and the difference of expression of the key genes of lipopeptide synthase between wild strain JSa-9 and the fusion strain was analyzed by real-time PCR. LI-F antibacterial lipopeptide yield of the fusion strain F5-15 was 3.1-fold of the original strain JSa9's, and the expression levels of the target genes were 10.48, 2.48, 2.1 and 11.8 fold of the initial strain JSa-9, respectively.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Protoplasts / Chromatography, High Pressure Liquid / Lipopeptides / Paenibacillus / Real-Time Polymerase Chain Reaction / Metabolism / Anti-Bacterial Agents Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Protoplasts / Chromatography, High Pressure Liquid / Lipopeptides / Paenibacillus / Real-Time Polymerase Chain Reaction / Metabolism / Anti-Bacterial Agents Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2015 Type: Article