Separation and cryopreservation of cord blood mononuclear cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 351-354, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-337623
ABSTRACT
The influencing factors on cord blood storage after collection and mononuclear cell separation as well as cryopreservation were studied. The mononuclear cell are separated from blood after blood collection, then cryopreserved and washed after thawed. Results showed that the cord blood kept at 4 degrees C or room temperature less than 24 hours after blood collection, mononuclear cell separated by hydroxyethylstarch and 2 centrifugations, mononuclear cell cryopreserved with 50% DMSO and autoplasma from cord blood as protectives and washing the cells after thawing. In conclusion, the optimal project in this study can effectively preserve cord blood mononuclear cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Blood Preservation
/
Leukocytes, Mononuclear
/
Cryopreservation
/
Cell Separation
/
Cell Biology
/
Fetal Blood
/
Methods
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2002
Type:
Article
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