Effects of linoleic acid on intracellular calcium concentration in primarily cultured rat pancreatic β-cells and underlying mechanism / 生理学报
Acta Physiologica Sinica
;
(6): 529-534, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-337716
ABSTRACT
In this study, we investigated the mechanism of linoleic acid-stimulated increase in intracellular calcium concentration ([Ca(2+)](i)) in pancreatic islet β-cells. Pancreatic islet cells were primarily isolated from rats and cultured for the experiments. The cells were loaded with Fluo-3/AM, the indicator of [Ca(2+)](i), and the intensity of Fluo-3 was measured using confocal microscope. The islet β-cells were identified by immunocytochemical staining with insulin antibody after recording. The drugs were given by perfusion system. The results showed that linoleic acid (20 μmol/L) stimulated [Ca(2+)](i) increase with the first peak increase and the following plateau increase. Linoleic acid-stimulated [Ca(2+)](i) increase was partly inhibited by removal of extracellular calcium and by transient receptor potential (TRP) channel blocker, La(3+), and it was totally blocked by exhaustion of intracellular calcium stores and inhibition of phospholipase C. It is concluded that linoleic acid stimulates [Ca(2+)](i) increase in islet β-cells through both extracellular calcium influx via TRP channels and calcium release from intracellular calcium stores.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Calcium
/
Rats, Sprague-Dawley
/
Linoleic Acid
/
Cell Biology
/
Insulin-Secreting Cells
/
Transient Receptor Potential Channels
/
Primary Cell Culture
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2010
Type:
Article
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