The molecular mechanism of apoptosis of human umbilical vein endothelial cells induced by monocyte chemotacitic protein-1 / 生理学报
Acta Physiologica Sinica
;
(6): 63-68, 2010.
Article
in English
| WPRIM
| ID: wpr-337778
ABSTRACT
The present study was aimed to investigate whether Bcl-2, Fas and Bax are involved in monocyte chemotacitic protein-1 (MCP-1)-induced apoptosis of human umbilical vein endothelial cells (hUVECs). hUVECs were cultured, and the purity was identified by immunofluorescence and immunohistochemistry with specific anti-von Willebrand factor (vWF) and anti-VEGF receptor-2 (KDR) antibodies. With 90% confluence hUVECs were serum-starved for 12 h, and then treated with different concentrations of MCP-1 (0.1, 1.0, 10, 100 ng/mL) for 24 and 48 h respectively. The expressions of apoptosis related proteins Fas, Bcl-2, Bax were detected by flow cytometry (FACS) and Western blot. As shown in our preliminary study, MCP-1 induced apoptosis of hUVECs in a dose-dependent manner at both 24 h and 48 h. FACS and Western blot analysis results in the present study indicated that MCP-1 promoted the expression of proapoptotic proteins Bax and Fas and inhibited the expression of antiapoptotic protein Bcl-2. These results suggest that MCP-1 may induce the apoptosis of hUVECs through evoking the imbalance between proapoptotic Fas/Bax and antiapoptotic Bcl-2 protein.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cells, Cultured
/
Apoptosis
/
Chemokine CCL2
/
Fas Receptor
/
Proto-Oncogene Proteins c-bcl-2
/
Cell Biology
/
Atherosclerosis
/
Bcl-2-Associated X Protein
/
Human Umbilical Vein Endothelial Cells
/
Metabolism
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Acta Physiologica Sinica
Year:
2010
Type:
Article
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