HRM identification of Chinese medicinal materials Mutong / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2304-2308, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-337940
ABSTRACT
High resolution melting (HRM), an important technology for genotyping and mutation scanning, has broad prospects in the authentification of traditional Chinese medicine. This paper selected universal trnH-psbA primers and used HRM to establish a new methods for identification of Akebia herbs. PCR was conduct at the annealing temperature of 58 degrees C and 35 cycles. The range of the DNA template concentration, the primer concentration and the Mg2+ ion concentration were further analyzed. The results showed the Tm values of Caulis Akebiae was (81.84 ± 0.16), (85.28 ± 0.16) degrees C and Caulis Clematidis Armandii was (83.22 ± 0.19) degrees C and Caulis Aristolochiae manshuriensis was (81.67 ± 0.14) degrees C, (84.24 ± 0.10) degrees C with 5-125 mg - L-' DNA template, 0.4 μmol x L(-1) primer, 2.0 mmol x L(-1) Mg2+. This method can achieve the authentification of Akebia herbs and is simple, fast, high-throughput, visual.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Chemistry
/
Chemistry Techniques, Analytical
/
Classification
/
DNA, Plant
/
Magnoliopsida
/
Transition Temperature
/
Genetics
/
Genotype
/
Methods
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Type:
Article
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