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Effect of Ligusticum wallichii-containing serum on expressions of Toll-like receptor 4 and myeloid differentiation factor 88 in hepatic stellate cells / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2191-2194, 2015.
Article in Chinese | WPRIM | ID: wpr-337961
ABSTRACT
To observe the effect of Ligusticum wallichii-containing serum on the expressions of Toll-like receptor 4 and myeloid differentiation factor 88 in hepatic stellate cells. Clean-grade SD rats were randomly divided into 5 groups and orally given L. wallichii decoction, colchicine and normal saline for 7 d to prepare L. wallichii-containing serums. Except for the blank group, all of the remaining groups were stimulated with LPS 1 mg x L(-1) for 24 h. After being intervened, the L. wallichii-containing serums were cultured in 5% CO2 incubator at 37 degrees C for 24 hours. The expression of TLR4 and MyD88 were detected by RT-PCR and Western blot. After HSC was stimulated with LPS, TLR4 and MyD88 mRNA and protein expressions were significantly higher than the blank control group (P < 0.01). After being intervened with L. wallichii-containing serum, TLR4 and MyD88 mRNA and protein expressions were notably lower than the model group (P < 0.05 or P < 0.01). In conclusion, L. wallichii-containing serum could regulate the TLR4 signaling pathway and show the anti-fibrosis effect by inhibiting the expression of TLR4 and MyD88 in LPS-induced HSCs.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Physiology / RNA, Messenger / Lipopolysaccharides / Rats, Sprague-Dawley / Ligusticum / Drug Therapy / Toll-Like Receptor 4 / Myeloid Differentiation Factor 88 / Hepatic Stellate Cells Type of study: Prognostic study Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Physiology / RNA, Messenger / Lipopolysaccharides / Rats, Sprague-Dawley / Ligusticum / Drug Therapy / Toll-Like Receptor 4 / Myeloid Differentiation Factor 88 / Hepatic Stellate Cells Type of study: Prognostic study Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article