Study on hepatotoxicity of aqueous extracts of Polygonum multiflorum in rats after 28-day oral administration: cholestasis-related mechanism / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2163-2167, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-337966
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of aqueous extracts of Polygonum multiflorum (AEPM) on bile acid synthesis, metabolism and transfer-related molecules in rat liver and the hepatotoxicity-related mechanism of P. multiflorum.</p><p><b>METHOD</b>Sprague-Dawley rats were orally administered with 30, 60 g x kg(-1) APEM once everyday for consecutively 28 days. At the end of the experiment, mRNA and protein expressions of hepatic MRP3, MRP2, BSEP, FXR and CYP7A1 were detected by Real-time PCR and Western blot</p><p><b>RESULT</b>Compared with the normal group, the AEPM high dose group showed significant increases in mRNA expressions of hepatic MRP3 and BSEP of male rats (P < 0.05); AEPM high and low dose groups revealed a notable decrease in mRNA expressions of hepatic FXR (P < 0.05) and remarkable rises in mRNA expressions of hepatic MRP3, MRP2, BSEP, CYP7A1 among female rats (P < 0.05). According to the test results of western blot assay, AEPM high and low dose groups showed consistent changes in protein and mRNA expressions hepatic MRP3, MRP2, BSEP, FXR, CYP7A1.</p><p><b>CONCLUSION</b>The 28 oral administration with AEPM in rats showed a certain effect on expressions of bile acid synthesis, metabolism and transfer-related proteins, as well as cholestatic or choleretic effects in the mRNA expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bile Acids and Salts
/
Plant Extracts
/
Cholestasis
/
Administration, Oral
/
Rats, Sprague-Dawley
/
Toxicity
/
Fallopia multiflora
/
Liver
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Type:
Article
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