Effect of 8-methoxypsoralen on PHA-induced DNA synthesis of human lymphocyte in the absence of UVA / 대한피부과학회지
Korean Journal of Dermatology
;
: 601-607, 1992.
Article
in Korean
| WPRIM
| ID: wpr-33798
ABSTRACT
We have observed the dark effect of 8-methoxypsoralen (8-MOP) on the viability and DNA synthesis in human lymphocyte cultures after stimulation with phytohemagglutinin (PHA) in the absence of ultraviolet A radiation. The concentrations of 8-MOP was 0.5-3.2 microgram/ml. We have also measured the LDH activity in supernatants of lymphocyte cultures treated with 8-MOP. The results were as follows 1. There was no 8-MOP dose-dependent decrease in the viability of lymphocytes up to 8MOP 32microgram/ml. 2. There was 8-MOP dose-dependent decrease in PHA-induced DNA synthesis of lymphocytes from the concentration of 8-MOP 2microgram/ml. 3. There was a time-dependent decrease in PHA-induced DNA synthesis of lymphocytes at the conscentration of 8-MOP 32microgram/ml. 4. There was no LDH release in supernatant of lymphocyte cultures after incubation with 8-MOP up to 8-MOP 32microgram/ml.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
Lymphocytes
/
Methoxsalen
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Dermatology
Year:
1992
Type:
Article
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