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Identification and bioinformatics analysis of genes associated with MVA pathway in Magnolia officinalis / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2077-2083, 2015.
Article in Chinese | WPRIM | ID: wpr-337980
ABSTRACT
Methyl valerate (MVA) pathway is one of the important ways for synthesis of terpenoids. This study was based on data of the transcriptome sequencing of Magnolia officinalis, the associated genes MoACOT, MoHMGS, MoHMGR, MoMK in methyl valerate (MVA) pathway, were completed in detail by using bioinformatics methods. The results of analysis showed that MoACOT and MoMK were stable hydrophobic proteins, MoHMGS and MoHMGR were unstable hydrophobic protein. The secondary structures of all proteins were hybrid architecture,and alpha helical were the major motifs. There were no clear transmembrane domains in MoACOT, MoHMGS and MoMK, but two transmembrane domains were founded in MoHMGR which were from 39-61 aa and 82-104 aa resepectively. The results of evolutionary relationship analysis showed that MoACOT, MoHMGS, MoHMGR and MoMK had relative close relationship to angiosperm or dicotyledonous plants, and accorded with genetic evolution rule. From transcriptome data, transcripted level of MoACOT, MoHMGS, MoHMGR, MoMK in M. officinalis and M. officinalis var. biloba was not significantly different. The result provided theoretical reference for study on Methyl valerate (MVA) pathway of terpenoid of M. officinalis.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Terpenes / Genes, Plant / Computational Biology / Magnolia / Genetics / Metabolism Type of study: Diagnostic study Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Terpenes / Genes, Plant / Computational Biology / Magnolia / Genetics / Metabolism Type of study: Diagnostic study Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article