Metabolites of Aspergillus fumigatus / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3083-3085, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-337988
ABSTRACT
Aspergillus fumigatus, a type of endophytic fungi from Erthrophleum fordii, was fermented with GPY culture medium. Fermented liquid and mycelium were extracted from fermented products after freezing and thawing treatment. After alcohol extraction, mycelium was extracted with ethyl acetate and n-butyl alcohol, respectively. According to the results of cytotoxity of tumor cells, ethyl acetate extracts were studied for their chemical constituents. Five diketopiperazine compounds were separated and purified with silica gel, MCI and Sephadex LH-20 column chromatography, reversed-phase chromatographic column and preparative HPLC, their structures were identified as cyclo- (R-Pro-R-Phe) (1), cyclo- (trans-4-OH-D-Pro-D-Phe) (2), cyclo- (R-Tyr-S-Ile) (3), cyclo-(R-Phe-S-Ile) (4), and cyclo-(R-Val-S-Tyr) (5) by using spectral methods.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Aspergillus fumigatus
/
Chemistry
/
Mycelium
/
Cell Line, Tumor
/
Diketopiperazines
/
Endophytes
/
Fabaceae
/
Metabolism
/
Microbiology
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2012
Type:
Article
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