Significance of the changes of growth hormone receptor mRNA in liver after cardiopulmonary bypass in a rat model / 中华外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the significance of the changes of growth hormone receptor mRNA (GHR mRNA) in liver after cardiopulmonary bypass (CPB) in a rat model.</p><p><b>METHODS</b>The rat model of CPB was established and plasma samples were collected at different intervals. Growth hormone (GH), GH binding protein (GHBP), endotoxin, interleukin (IL)-6, prealbumin and liver function were determined respectively. The liver tissues were collected for the detection of the GHR mRNA by RT-PCR analysis.</p><p><b>RESULTS</b>Both the lesion of liver function and the rising of the concentrations of endotoxin and IL-6 were obviously at 1 h after CPB, and increased markedly 2 h after CPB. However, the changes of concentrations of GH and GHBP, the expressions of GHR mRNA were opposite. In addition, the expression of GHR mRNA, concentrations of GH and GHBP had a significant negative correlation to the concentration of bilirubin, endotoxin respectively (P < 0.01), and a positive correlation to prealbumin (P < 0.01).</p><p><b>CONCLUSIONS</b>The liver injury and downregulation of GH-GHR axis obviously exist during early stage of CPB because of the GHR mRNA expression inhibition resulting from endotoxemia. It may be one of the most important factors intervening the pathophysiology changes of liver during CPB.</p>