Proteomics technology for screening biomarkers of oligospermic candidates / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To screening specific biomarkers of oligospermic candidates from seminal plasma by proteomics technology.</p><p><b>METHODS</b>The protein spectra of 20 seminal plasma from oligospermic patients and healthy male individuals were analyzed by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) to compare different protein expressions.</p><p><b>RESULTS</b>Compared with normal control group, a specific protein peak of molecular weight 11 022.9 in the protein spectra of oligospermic patients'semen on H4 protein-chip reduced significantly, but the protein peak of molecular weight 11 507.4 elevated apparently. For SAX-2 protein-chip, a specific protein peak of molecular weight 16 751.6 in the protein spectra of oligospermic patients' semen elevated significantly.</p><p><b>CONCLUSION</b>Screening specific proteins in seminal plasma of oligospermic patients is important for understanding the mechanism of oligospermia and screening suspected oligospermic individuals.</p>