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Transplantation of human bone marrow-derived mesenchymal stem cells transfected with ectodysplasin for regeneration of sweat glands / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 2260-2268, 2011.
Article in English | WPRIM | ID: wpr-338560
ABSTRACT
<p><b>BACKGROUND</b>Patients with severe full-thickness burn injury suffer from their inability to maintain body temperature through perspiration because the complete destructed sweat glands can not be regenerated. Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent an ideal stem-cell source for cell therapy because of their easy purification and multipotency. In this study, we attempted to induce human BM-MSCs to differentiate into sweat gland cells for sweat gland regeneration through ectodysplasin (EDA) gene transfection.</p><p><b>METHODS</b>The dynamic expression of EDA and EDA receptor (EDAR) were firstly observed in the sweat gland formation during embryological development. After transfection with EDA expression vector, human BM-MSCs were transplanted into the injured areas of burn animal models. The regeneration of sweat glands was identified by perspiration test and immunohistochemical analysis.</p><p><b>RESULTS</b>Endogenous expression of EDA and EDAR correlated with sweat gland development in human fetal skin. After EDA transfection, BM-MSC acquired a sweat-gland-cell phenotype, evidenced by their expression of sweat gland markers by flow cytometry analysis. Immunohistochemical staining revealed a markedly contribution of EDA-transfected BM-MSCs to the regeneration of sweat glands in the scalded paws. Positive rate for perspiration test for the paws treated with EDA-transfected BM-MSCs was significantly higher than those treated with BM-MSCs or EDA expression vector (P < 0.05).</p><p><b>CONCLUSIONS</b>Our results confirmed the important role of EDA in the development of sweat gland. BM-MSCs transfected with EDA significantly improved the sweat-gland regeneration. This study suggests the potential application of EDA-modified MSCs for the repair and regeneration of injured skin and its appendages.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Sweat Glands / Bone Marrow Cells / Immunohistochemistry / Transfection / Cells, Cultured / Blotting, Western / Reverse Transcriptase Polymerase Chain Reaction / Cell Biology / Mesenchymal Stem Cell Transplantation / Cell Proliferation Limits: Animals / Female / Humans / Male / Pregnancy Language: English Journal: Chinese Medical Journal Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Sweat Glands / Bone Marrow Cells / Immunohistochemistry / Transfection / Cells, Cultured / Blotting, Western / Reverse Transcriptase Polymerase Chain Reaction / Cell Biology / Mesenchymal Stem Cell Transplantation / Cell Proliferation Limits: Animals / Female / Humans / Male / Pregnancy Language: English Journal: Chinese Medical Journal Year: 2011 Type: Article