The study of 3-dimensional structures of IgG with atomic force microscopy / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 277-282, 2005.
Article
in English
| WPRIM
| ID: wpr-338598
ABSTRACT
<p><b>OBJECTIVE</b>To detect 3-dimensional images of anti-N-methyl-D-aspartate receptor Nr1 (NMDAr1) polycolonal IgG affixed on mica in physiological environment.</p><p><b>METHODS</b>The images and data were obtained from a contact mode and commercial Si3N4 probed tip by using atomic force microscope (AFM).</p><p><b>RESULTS</b>The anti-NMDAr1 polycolonal IgG has a characteristic structure described as an ellipse spherical shape of 136.4 A x 62.8 A x 26.1 A. On the section of the ellipse edge there were two peaks about 13 nm in width.</p><p><b>CONCLUSIONS</b>Using AFM to investigate biomacromolecule can make us deeply understand the structure of IgG, which will instruct us to detect the membrane receptor protein as a labelling agent.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunoglobulin G
/
Chemistry
/
Receptors, N-Methyl-D-Aspartate
/
Gold Colloid
/
Microscopy, Atomic Force
/
Adsorption
/
Imaging, Three-Dimensional
/
Aluminum Silicates
/
Methods
Language:
English
Journal:
Chinese Journal of Traumatology
Year:
2005
Type:
Article
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