Expression and effect of Caspase-3 in neurons after tractive spinal cord injury in rats / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 220-224, 2005.
Article
in English
| WPRIM
| ID: wpr-338609
ABSTRACT
<p><b>OBJECTIVE</b>To investigate Caspase-3 expression and its role in neuronal apoptosis.</p><p><b>METHODS</b>The T13-L2 spinal cord of rats was injured by traction after the amplitude of P1-N1 wave, monitored by a cortical somatosensory evoked potential (CSEP) monitor, decreased to seventy percent of that before operation. Then rats were killed in 6 h, 1 d, 4 d, 7 d, 14 d and 21 d respectively after operation. Flow cytometer terminal deoxynucleotldyl transferease-mediated biotinylated deoxynuridine triphosphate nick end labeling (TUNEL), Caspase-3 activity assay and immunohistochemical method were applied to investigate Caspase-3 expression in the spinal cord tissue and to study neuronal apoptosis in rats.</p><p><b>RESULTS</b>After spinal cord injury, apoptotic cells detected by flow cytometry and TUNEL-positive cells were significantly more, and positive immunohistochemical staining of Caspase-3 and Caspase-3 activity were significantly higher in Group injury than in Groups control and laminectomy, respectively (P > 0.05, P > 0.01). Similar trend of changes was noticed in apoptotic cells, TUNEL-positive cells and positive immunohistochemical staining of Caspase-3, all of which reached their respective peak 7 days after operation. Caspase-3 activity reached its peak, however, 4 days postoperatively.</p><p><b>CONCLUSIONS</b>Increased expression and activity of Caspase-3 protein in neurons after tractive spinal cord injury is the biochemical signal of early spinal cell apoptosis. It is of great significance for understanding the mechanism of spinal cord injury.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Spinal Cord Injuries
/
Immunohistochemistry
/
Rats, Sprague-Dawley
/
Apoptosis
/
Caspases
/
In Situ Nick-End Labeling
/
Caspase 3
/
Flow Cytometry
Limits:
Animals
Language:
English
Journal:
Chinese Journal of Traumatology
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS