Construction of eukaryotic expression vector with brain-derived neurotrophic factor receptor trkB gene / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 142-146, 2005.
Article
in English
| WPRIM
| ID: wpr-338626
ABSTRACT
<p><b>OBJECTIVE</b>To construct an eukaryotic expression vector carrying rat brain-derived neurotrophic factor receptor trkB gene.</p><p><b>METHODS</b>Using the total RNA isolated from rat brain as template, the trkB gene was amplified by reverse-transcription-polymerase chain reaction (RT-PCR) with a pair of specific primers which contained the restrictive sites of EcoR I and BamH I. The amplified fragment of trkB gene was digested with EcoR I and BamH I, and then subcloned into cloning vector pMD18-T and expression vector pEGFP-C2 respectively. The recombinant plasmids were identified by restriction endonuclease enzyme analysis and PCR.</p><p><b>RESULTS</b>The amplified DNA fragment was about 1461 bp in length. Enzyme digestion and PCR analysis showed that the gene of trkB had been successfully cloned into vector pMD18-T and pEGFP-C2.</p><p><b>CONCLUSIONS</b>The trkB gene of rat has been amplified and cloned into the eukaryotic expression vector pEGFP-C2.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Schwann Cells
/
Templates, Genetic
/
RNA
/
Transfection
/
Genetic Therapy
/
Gene Expression Regulation
/
Sensitivity and Specificity
/
Cloning, Molecular
/
Rats, Wistar
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Chinese Journal of Traumatology
Year:
2005
Type:
Article
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