Labeling of human insulin-like growth factor-I eukaryotic expression vector with green fluorescent protein / 中华创伤杂志(英文版)

ABSTRACT

<p><b>OBJECTIVE</b>To label human insulin-like growth factor-I (hIGF-I) eukaryotic expression vector with green fluorescent protein (GFP) for the repair of articular cartilage defects.</p><p><b>METHODS</b>GFP cDNA was inserted into pcDNA(3.1)-hIGF-1 to construct the co-expression vector with two multiple cloning sites mammalian expression vector under two cytomegalovirus promoters/enhancers respectively. Recombinant pcGI was transfected into NIH 3T3 cells with the help of lipofectamine.</p><p><b>RESULTS</b>Enzyme digestion and agarose gel electrophoresis analysis revealed that pcGI vector contained correct GFP and hIGF-I cDNA. Expression of hIGF-1 and GFP was confirmed in transfected NIH 3T3 cells by immunocytochemical analysis and fluorescence microscopy.</p><p><b>CONCLUSIONS</b>hIGF-I eukaryotic expression vector has been successfully labeled with GFP.</p>