The effects of sodium hyaluronate on mRNA expressions of matrix metalloproteinase-1, -3 and tissue inhibitor of metalloproteinase-1 in cartilage and synovium of traumatic osteoarthritis model / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 8-12, 2005.
Article
in English
| WPRIM
| ID: wpr-338654
ABSTRACT
<p><b>OBJECTIVE</b>To observe the influence of intra-articular injection of sodium hyaluronate (HA) on the mRNA expressions of matrix metalloproteinase-1,-3 (MMP-1,-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in cartilage and synovium of traumatic osteoarthritis (OA).</p><p><b>METHODS</b>Sixteen white rabbits underwent unilateral anterior cruciate ligament transection (ACLT) were divided into 2 groups randomly 5 weeks after transection. The experimental group rabbits received 0.3 ml of 1% HA by intra-articular injection once a week. Animals in the control group were treated under the same conditions using physiological saline. Ten weeks following surgery, cartilage and synovium were harvested. The mRNA expressions of MMP-1, MMP-3 and TIMP-1 were analyzed using reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>In synovium, the mRNA expression of MMP-3 was suppressed in the HA injection group. HA treatment had no effect on the MMP-3 expression in cartilage. No significant difference of MMP-1 and TIMP-1 expressions in cartilage and synovium was found between the HA injection group and the control group.</p><p><b>CONCLUSIONS</b>One of the mechanisms of the therapeutic effect of HA may be the inhibition of expression of MMP-3 in synovium during early stage of traumatic OA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Synovial Membrane
/
RNA, Messenger
/
Cartilage, Articular
/
Anterior Cruciate Ligament
/
Matrix Metalloproteinase 3
/
Tissue Inhibitor of Metalloproteinase-1
/
Osteoarthritis, Knee
/
Reverse Transcriptase Polymerase Chain Reaction
Limits:
Animals
Language:
English
Journal:
Chinese Journal of Traumatology
Year:
2005
Type:
Article
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