Analysis of polymorphisms in the promoter region of interleukin-1beta by restriction fragment length polymorphism-PCR / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 271-274, 2004.
Article
in English
| WPRIM
| ID: wpr-338677
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the frequencies of -1470, -511 and -31 single nucleotide polymorphisms (SNPs) in the promoter of IL-1beta and its haplotype constitution in Chongqing population.</p><p><b>METHODS</b>One hundred and twelve healthy Chongqing people were enrolled in this study. Polymorphisms at -1470 (G to C), -511 (T to C) and -31 (C to T) of IL-1beta were genotyped with the method of restriction fragment length polymorphism (RFLP). Haplotype frequencies were analyzed by Arlequine software.</p><p><b>RESULTS</b>Frequencies of IL-1beta -1470, -511 and -31 SNPs were 41.67%, 50% and 45.33%, respectively. Genotype frequencies of -1470 locus were 39.81%, 37.04% and 23.15% for G/G, G/C and C/C respectively. As for T-511C SNP, genotype frequencies of T/T, T/C and C/C were 29.91%, 40.18% and 29.91%, respectively. Genotyping results of C/C, C/T, and T/T of -31 locus were 35.51%, 38.32% and 26.71% respectively. Haplotype analysis found that there were mainly three haplotypes constituted by three SNPs, ie., G-T-C, C-T-C and G-C-T.</p><p><b>CONCLUSIONS</b>Polymorphisms exist in the promoter of IL-1beta in Chongqing population. Three SNPs locate in the same haplotype block.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Haplotypes
/
Polymorphism, Restriction Fragment Length
/
China
/
Polymerase Chain Reaction
/
Cohort Studies
/
Sensitivity and Specificity
/
Interleukin-1
/
Promoter Regions, Genetic
/
Polymorphism, Single-Stranded Conformational
/
Asian People
Type of study:
Diagnostic study
/
Etiology study
/
Incidence study
/
Observational study
/
Risk factors
Limits:
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
English
Journal:
Chinese Journal of Traumatology
Year:
2004
Type:
Article
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