Analysis of genetic diversity and genetic relationships of Lonicera macranthoides cultivars / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study genetic diversity and genetic relationships among Lonicera macranthoides cultivars.</p><p><b>METHOD</b>Five cultivars were estimated by ISSR and SRAP. The data of amplified bands were analyzed by Treeconw software. The system diagram of genetic relationship was built by UPGMA.</p><p><b>RESULT</b>Twenty ISSR primers amplified 186 bands with 103 (54.63%) polymorphic bands and 58 SRAP primer combinations amplified 591 bands with 347(55.46%) polymorphic bands. Genetic distance ranges were 0.058 4-0.230 8 (by ISSRs) and 0.1071-0.2611 (by SRAPs). Both ISSR and SRAP analyses revealed a middle level of genetic diversity in L. macranthoides cultivars. The dendrograms based on SRAP and ISSR markers were not all the same.</p><p><b>CONCLUSION</b>The genetic diversity of L. macranthoides cultivars is middle. ISSR and SRAP markers can be effectively applied to genetic analysis in L. macranthoides cultivars.</p>