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Study on molecular characteristics of four components contained in Hedysari Radix polysaccharide by gel permeation chromatography-multiangle laser light scattering technology (GPC-MALLS) / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 1798-1803, 2012.
Article in Chinese | WPRIM | ID: wpr-338759
ABSTRACT
<p><b>OBJECTIVE</b>To determine such molecular characteristic parameters as absolute molecular weight, molecular weight distribution, root-mean-square turning radius (Rg) and polydispersity index (Mw/Mn) of four components contained in Hedysari Radix polysaccharide 3 (HPS-3) and map weight-average molecular weight (Mw) with root-mean-square turning radius (Rg), in order to calculate conformations of the four components at solution state.</p><p><b>METHOD</b>The gel permeation chromatography-multi angle laser light scatting (GPC-MALLS) was adopted, with 0.1 mol x L(-1) NaNO3 contained 0.02% NaN3 as the mobilephase, Ultrahydrogel 1000 connected in series with Ultrahydrogel500.</p><p><b>RESULT</b>Among the four components of HPS-3, HPS-3-C showed the highest weight average molecular weight of 1.986 x 10(5) g x mol(-1), followed by HPS-3-B 1.113 x 10(5) g x mol(-1) and HPS-3-D 8.457 x 10(4) g x mol(-1) HPS-3-A showed the lowest weight average molecular weight of 1. 223 x 10(4) g x mol(-1) but the highest square radius of gyration, that is 55.5 nm. HPS-3-D had the widest range of molecular weight distribution in four components, with the polydispersity index (Mw/Mn) of 2.543. In the mobile phase, HPS-3-A was globular structure, HPS-3-C was random coil, HPS-3-B and HPS-3-D were both highly branched structure.</p><p><b>CONCLUSION</b>The results provided necessary basis for further studies on molecular characteristics of the four components contained in HPS-3 and their relationship with bioactivity.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Polysaccharides / Scattering, Radiation / Drugs, Chinese Herbal / Chemistry / Chromatography, Gel / Lasers / Light Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Polysaccharides / Scattering, Radiation / Drugs, Chinese Herbal / Chemistry / Chromatography, Gel / Lasers / Light Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2012 Type: Article