An infrared imaging system for detecting electrophoretic mobility shift of DNA-protein complexes / 南方医科大学学报
Journal of Southern Medical University
;
(12): 289-291, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-339007
ABSTRACT
<p><b>OBJECTIVE</b>To establish a new non-radioactive method for electrophoretic mobility shift assay (EMSA) to investigate the binding between glucocorticoid induced leucine zipper (GILZ) and peroxisome proliferator-activated receptor-gamma 2 (PPARgamma2) promoter oligonucleotides.</p><p><b>METHODS</b>GILZ protein prepared by prokaryotic expression was linked to PPARgamma2 promoter oligonucleotides end-labeled with IRDye 800 infrared dye. The DNA-protein complex was separated with non-denatured polyacrylamide gel and scanned with the Odyssey. Infrared Imaging System.</p><p><b>RESULTS</b>One lane of DNA-protein complex was clearly presented, and the signal intensity increased along with the increment of the protein load.</p><p><b>CONCLUSION</b>This infrared imaging system can be used for EMSA for detecting the DNA-protein complex with high sensitivity efficiency and allows easy operation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Protein Binding
/
Binding Sites
/
DNA
/
Proteins
/
Chemistry
/
Gene Expression Regulation
/
Electrophoretic Mobility Shift Assay
/
DNA-Binding Proteins
/
Protein Interaction Domains and Motifs
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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