Epithelial sodium channel subunit mRNA expression in acutely isolated rat alveolar type II cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 54-56, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-339067
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the epithelial sodium channel (ENaC) subunit mRNA expression in acutely isolated rat alveolar type II (ATII) cells.</p><p><b>METHODS</b>Acutely isolated ATII cells from 20 SD rats were purified and ENaC alpha, beta, gamma-subunit mRNA levels were determined by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>mRNA expressions of all the subunits were detected in the ATII cells, and ENaC alpha-subunit mRNA showed significantly higher expression than beta- and gamma-subunit mRNAs, and the expressions of the latter two mRNAs were comparable.</p><p><b>CONCLUSION</b>As the predominant ENaC subunit expressed at the mRNA level in rat ATII cells, the alpha-subunit of ENAC plays an important role in alveolar fluid clearance.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pulmonary Alveoli
/
RNA, Messenger
/
Cells, Cultured
/
Rats, Sprague-Dawley
/
Cell Biology
/
Epithelial Sodium Channels
/
Genetics
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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