Effect of AT-1 alpha receptor gene silencing on nuclear factor-kappaB activity in hepatic Kupffer cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 20-22, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-339077
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of angiotensin II type-1 (AT-1) alpha receptor gene silencing on nuclear factor-kappaB (NF-kappaB) activity in hepatic Kupffer cells.</p><p><b>METHODS</b>The expression of AT-1 alpha receptors in primary isolated cultured hepatic Kupffer cells was detected by immunohistochemistry. pSilencer/AT-1 alpha receptor siRNA plasmids were transfected into Kupffer cells, which were subsequently exposed to 10(-6) mol/L angiotensin II (Ang II) for 60 min. The changes in the DNA binding activity of NF-kappaB in the cells was assessed using electrophoretic gel mobility shift assay (EMSA).</p><p><b>RESULTS</b>AT-1 alpha receptor expression was detected in Kupffer cells. NF-kappaB DNA binding activity was markedly increased in Kupffer cells after Ang II stimulation, and obviously inhibited by transfectiom with pSilencer/AT-1 alpha receptor siRNA plasmid.</p><p><b>CONCLUSION</b>Ang II stimulation of Kupffer cell results in increased activation of NF-kappaB via AT-1 alpha receptor.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cells, Cultured
/
NF-kappa B
/
Cell Biology
/
RNA, Small Interfering
/
RNA Interference
/
Receptor, Angiotensin, Type 1
/
Genetics
/
Kupffer Cells
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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