The biological activity of MHC classII transactivator ribonuclease P: a novel approach for hepatic transplantation rejection / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 745-748, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-339094
ABSTRACT
<p><b>OBJECTIVE</b>This paper studied the effect of RNaseP against CIITA on repressing class II MHC (MHCII) expression.</p><p><b>METHODS</b>It was constructed that M1-RNA with guide sequences (GS), recognizing the 629 site of CIITA (M1-629-GS), by PCR from pTK117 plasmid, then was cloned into psNAV (psNAV-M1-629-GS). CIITA target gene was obtained from Raji cell by RT-PCR, and then inserted into pGEM-7zf (+) (pGEM-800). psNAV-M1-629-GS and pGEM-800 were transcribed and then mixed up and incubated in vitro. Stable transfectants of hepatocyte with psNAV-M1-629-GS by nanometer were tested for MHCII induction by recombinant human interferon-gamma (IFN-gamma). mRNA abundance of CIITA was measured by RT-PCR.</p><p><b>RESULTS</b>It showed that M1-629-GS could exclusively cleave pGEM-800 that formed a base pair with the GS. When induced with IFN-gamma, the expression of HLA-DR, -DP, -DQ on psNAV-M1-629-GS+ hepatocyte was (1.01+/-0.51)%, (4.37+/-1.28)%, (1.98+/-0.42)% respectively, was down-modulated 90.65%, 89.11% and 65.32% compared with control, while the mRNA content of CIITA reduced significantly (P<0.01).</p><p><b>CONCLUSION</b>M1-629-GS could effectively repress MHCII expressing through cleaving CIITA mRNA. These results provided insight into the future application of it as a new nucleic acid drug against the rejection of hepatic transplantation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
RNA, Messenger
/
Nuclear Proteins
/
Histocompatibility Antigens Class II
/
Trans-Activators
/
Liver Transplantation
/
Ribonuclease P
/
Allergy and Immunology
/
Genetics
/
Graft Rejection
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2003
Type:
Article
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