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Changes of alkaline phosphatase sugar chains in hepatocellular carcinoma tissue / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 739-741, 2003.
Article in Chinese | WPRIM | ID: wpr-339096
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes of sugar chain structures of alkaline phosphatase (ALP) in hepatoma tissue and its relation to the invasiveness of hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>The binding ratios of ALP from 9 normal liver tissues, 16 hepatoma tissues and 16 noncancerous tissues surrounding hepatoma were analysed by affinity chromatography on various lectin columns including leukoagglutinating phytohemagglutinin (L-PHA), lentil lectin (LCA), Datura stramonium agglutinin (DSA), erythroagglutinating phytohemagglutinin (E-PHA) and Sambucus nigra bark agglutinin (SNA).</p><p><b>RESULTS</b>The binding ratios of ALP on L-PHA (22.94%+/-5.30%), DSA (55.97%+/-13.72%), LCA (38.16%+/-8.87%), E-PHA (11.56%+/-4.81%) and SNA (69.80%+/-13.71%) in HCC tissues were significantly increased (P<0.01) compared with that in normal liver tissues (L-PHA 5.89%+/-2.75%, DSA 36.20%+/-11.58%, LCA 17.90%+/-6.71%, E-PHA 5.38%+/-2.20%, SNA 57.32%+/-11.27%), respectively. t values between the two groups were 8.94, 3.64, 5.94, 3.62 and 2.32, respectively. L-PHA-binding ratio (25.84%+/-4.67%) of ALP in HCC with invasiveness was significantly higher than that (18.10%+/-3.64%) without invasiveness (t=3.71, P<0.01).</p><p><b>CONCLUSION</b>The changes of ALP sugar chain structures occur in HCC tissue. b1-6 branching sugar chain structure of ALP is related to the invasiveness of HCC.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Carbohydrates / Chemistry / Chromatography, Affinity / Carcinoma, Hepatocellular / Alkaline Phosphatase / Lectins / Liver Neoplasms / Metabolism / Neoplasm Invasiveness Limits: Humans Language: Chinese Journal: Chinese Journal of Hepatology Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Carbohydrates / Chemistry / Chromatography, Affinity / Carcinoma, Hepatocellular / Alkaline Phosphatase / Lectins / Liver Neoplasms / Metabolism / Neoplasm Invasiveness Limits: Humans Language: Chinese Journal: Chinese Journal of Hepatology Year: 2003 Type: Article