Study on the angiogenic ability of tumor fibroblasts / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 735-738, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-339097
ABSTRACT
<p><b>OBJECTIVES</b>To explore the role of fibroblasts derived from tumor in the tumor angiogenesis.</p><p><b>METHODS</b>Two-well co-culture system were used to detect the expression of MMP-9, TGF-beta1, TN and bcl-2 in L929-H22 cells, and their ability of promoting angiogenesis of ECV304 cells and invasion of MDA-MB-231 cells respectively, which were established in our laboratory before. Then their adhesion and the effect of their supernatant on H22 cells proliferation were analysed.</p><p><b>RESULTS</b>Compared with L929 cells, the adhesion potential of L929-H22 cells increased (F>or=104.32, P<0.001), with the higher level of expression of MMP-9, bcl-2, TN, and TGF-beta1 in L929-H22 cells in creased (t>or=3.3055, P<0.01). L929-H22 and L929 cells enhanced the invasiveness of human mammary cancer MDA-MB-231 cells through artificial basement membrane (Matrigel) 1.21 and 0.48 times respectively (F=266.3, P<0.001). L929-H22 cells induced morphogenesis of ECV304 cells. L929-H22 stimulated endothelial cells to form more and longer tubes than L929 did (F>or=23.75, P<0.01). 25% CM of L929-H22 cells stimulated the growth of H22 cells (F=266.30, P<0.05).</p><p><b>CONCLUSION</b>The results suggested that fibroblasts in tumors secrete more growth factors and angiogenic factors to promote the angiogenesis and invasion of solid tumors.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Transforming Growth Factor beta
/
Proto-Oncogene Proteins c-bcl-2
/
Matrix Metalloproteinase 9
/
Cell Line, Tumor
/
Fibroblasts
/
Neoplasm Invasiveness
/
Neoplasms
/
Neovascularization, Pathologic
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2003
Type:
Article
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