The primary study on the anti-HBV effect of whole recombinant yeast / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVES</b>Based on the immunologic character of Pichia pastoris yeast, a new therapeutic vaccine, whole recombinant yeast, was used to explore a new way to activate cell-mediated anti-viral immunity.</p><p><b>METHODS</b>The recombinant plasmids, pPIC9K/S and PIC9K/hsp(1-370)-S, were constructed by inserting the gene encoding HBsAg, HSP70 (1-370) -HBsAg into vector pPIC9K and then the recombinants were transfected into Pichia pastoris yeast,GS115, respectively. Then that recombinant yeast immunized BALB/C mice were detected for humoral and cellular immunity to HBsAg.</p><p><b>RESULTS</b>Recombinant yeast successfully activated the humoral immunity to HBsAg in mice, but failed to activate the cellular immunity.</p><p><b>CONCLUSION</b>The whole recombinant yeast can be used as vaccine, but need further study for optimal way of immunization.</p>