Differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 673-675, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-339123
ABSTRACT
<p><b>OBJECTIVE</b>To observe the differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment into hepatocytes.</p><p><b>METHODS</b>Rat hepatic fibrosis was induced by subcutaneous injection of CCl4. Bone marrow stem cells with Thy positive, CD3 and CD45RA negative were enriched from the bone marrow by fluorescence-activated cell sorting. The bone marrow stem cells were labeled with PKH26-GL, and then autotransplanted. After six weeks, albumin, ck8 and a-smooth muscle actin expression were determined by immunocytochemistry.</p><p><b>RESULTS</b>The PKH26-GL labeled cells expressed albumin and ck8, but did not express a-smooth muscle actin in hepatic fibrogenesis environment.</p><p><b>CONCLUSION</b>Bone marrow stem cells in hepatic fibrogenesis environment can differentiate into hepatocytes, but can't differentiate into myofibroblasts</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Bone Marrow Cells
/
Hematopoietic Stem Cells
/
Cell Differentiation
/
Rats, Sprague-Dawley
/
Hepatocytes
/
Environment
/
Liver Cirrhosis
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2003
Type:
Article
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