Inhibitive effects of recombinant adenovirus-mediated human endostatin on the growth of human hepatocellular carcinoma xenograft in nude mice / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 542-545, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-339183
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibitive effect on the growth of hepatocellular carcinoma (HCC) xenografted in nude mice by adenovirus-mediated human endostatin gene.</p><p><b>METHODS</b>The expression efficiency of endostatin was examined after ECV-304 cells infected with Ad/hEndo by western blot. The hepatoma BEL-7402 cells were injected into Balb/c nude mice to detect the inhibition of Ad/hEndo on the growth of HCC xenografted in nude mice. The expression of endostatin mRNA in tumor tissue was analyzed with RT-PCR, and its distribution in vivo was also analyzed.</p><p><b>RESULTS</b>High level expression of endostatin achieved in infected ECV-304 cells by western blot. Ad/hEndo significantly inhibited the growth of xenografted BEL-7402 tumors (F=4.061, P<0.05). The intratumoral microvessel density (MVD) decreased significantly in the treated mice (6.88+/-1.08 vs 13.60+/-1.71, t=9.216, P<0.01). The expression of endostatin mRNA in tumor tissue was detected by RT-PCR in 3 days after administration intratumorally with Ad/hEndo and almost disappeared in 7 days. Endostatin mRNA was mainly located in tumor tissue with a higher concentration than that in heart, lung, spleen and liver after Ad/hEndo administration.</p><p><b>CONCLUSION</b>Adenovirus-mediated human endostatin gene can be expressed efficiently in vitro and in vivo, and significantly inhibit the growth of BEL-7402 xenografted tumors in nude mice.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Transplantation, Heterologous
/
RNA, Messenger
/
Pharmacokinetics
/
Tissue Distribution
/
Genetic Therapy
/
Adenoviridae
/
Reverse Transcriptase Polymerase Chain Reaction
/
Endostatins
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2003
Type:
Article
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