Effects of reducing FasL expression on T cell apoptosis in lung cancer cell line H460 via siRNA technology / 中国肺癌杂志

ABSTRACT

<p><b>BACKGROUND</b>Lung cancer cells can upregulate the expression of Fas ligand (FasL) and counterattack tumor-infiltration lymphocyte (TIL) expressing Fas via the FasL/Fas pathway, therefore escape from immunosurveillance and impair local anti-tumor immune capacity. The aim of this study is to investigate the effects of reducing FasL expression on T cell apoptosis in lung cancer cell line H460 via small interfering RNA (siRNA) technology.</p><p><b>METHODS</b>In vitro chemically synthesized siRNA targeting FasL as well as constructed plasmid vector were transfected into H460 cells, wherein the interfering effect and alterations in T cell apoptosis were observed.</p><p><b>RESULTS</b>Sequence-specific interfering effect was detected at RNA and protein levels by RT-PCR and Western blot in the H460 si group, and the reduction of FasL expression was capable of rescuing T cell apoptosis induced by lung cancer cells.</p><p><b>CONCLUSIONS</b>FasL can be utilized as a new target in gene therapy of lung cancer.</p>