Expression and value of hnRNP B1 in sputum of patients with lung cancer by immunohistochemistry method / 中国肺癌杂志

ABSTRACT

<p><b>BACKGROUND</b>The expression of heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 increases significantly in lung cancer tissues. The aim of this study is to investigate the expression of hnRNP B1 in exfoliative cells in the sputum of patients with lung cancer and its sensitivity and specificity in diagnosis of lung cancer.</p><p><b>METHODS</b>The expression of hnRNP B1 in sputum was detected by immunohistochemistry (IHC) using anti-hnRNP B1 monoclonal antibody in 70 patients with lung cancer and 30 normal subjects (control group).</p><p><b>RESULTS</b>The results of sputum exfoliative cytology were 27 positive in 70 samples diagnosed with lung cancer, whose sensitivity and specificity were 38.6% and 100.0% respectively. The results of sputum immunohistochemistry were 50 positive in 70 samples, with 23 positive in 29 squamous cell carcinoma and 17 positive in 26 adenocarcinoma and 10 positive in 15 small cell lung carcinoma. Sputum IHC of hnRNP B1 gave a sensitivity of 71.4% and specificity of 93.3%. Sputum IHC of hnRNP B1 was more sensitive than sputum exfoliative cytology (P < 0.05).</p><p><b>CONCLUSIONS</b>hnRNP B1 overexpresses in sputum exfoliative cells and its sensitivity is better than sputum exfoliative cytology in diagnosis of lung cancer.</p>