The structure, expression and function prediction of DAZAP2, a down-regulated gene in multiple myeloma / 基因组蛋白质组与生物信息学报·英文版
Genomics, Proteomics & Bioinformatics
; (4): 47-54, 2004.
Article
in En
| WPRIM
| ID: wpr-339495
Responsible library:
WPRO
ABSTRACT
In our previous studies, DAZAP2 gene expression was down-regulated in untreated patients of multiple myeloma (MM). For better studying the structure and function of DAZAP2, a full-length cDNA was isolated from mononuclear cells of a normal human bone marrow, sequenced and deposited to Genbank (AY430097). This sequence has an identical ORF (open reading frame) as the NM_014764 from human testis and the D31767 from human cell line KG-1. Phylogenetic analysis and structure prediction reveal that DAZAP2 homologues are highly conserved throughout evolution and share a polyproline region and several potential SH2/SH3 binding sites. DAZAP2 occurs as a single-copy gene with a four-exon organization. We further noticed that the functional DAZAP2 gene is located on Chromosome 12 and its pseudogene gene is on Chromosome 2 with electronic location of human chromosome in Genbank, though no genetic abnormalities of MM have been reported on Chromosome 12. The ORF of human DAZAP2 encodes a 17-kDa protein, which is highly similar to mouse Prtb. The DAZAP2 protein is mainly localized in cytoplasm with a discrete pattern of punctuated distribution. DAZAP2 may associate with carcinogenesis of MM and participate in yet-to-be identified signaling pathways to regulate proliferation and differentiation of plasma cells.
Full text:
1
Index:
WPRIM
Main subject:
Phylogeny
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Chromosomes, Human, Pair 2
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Chromosomes, Human, Pair 12
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Molecular Sequence Data
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Base Sequence
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Pseudogenes
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Down-Regulation
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Likelihood Functions
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Sequence Alignment
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Amino Acid Sequence
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Genomics, Proteomics & Bioinformatics
Year:
2004
Type:
Article