The epitope study on the SARS-CoV nucleocapsid protein / 基因组蛋白质组与生物信息学报·英文版
Genomics, Proteomics & Bioinformatics
;
(4): 198-206, 2003.
Article
in English
| WPRIM
| ID: wpr-339506
ABSTRACT
The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA), the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins, whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptide Fragments
/
Plasmids
/
Recombinant Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Chemistry
/
Blotting, Western
/
Nucleocapsid Proteins
/
Severe acute respiratory syndrome-related coronavirus
/
Allergy and Immunology
/
Genetics
Limits:
Humans
Language:
English
Journal:
Genomics, Proteomics & Bioinformatics
Year:
2003
Type:
Article
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