Effects of genistein on proliferation and differentiation of osteoblast / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 182-184, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-339645
ABSTRACT
<p><b>AIM</b>To study the effects of genistein on proliferation and differentiation of osteoblasts in neonatal rat calvaria cultures.</p><p><b>METHODS</b>Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion, and cultured in the presence of different doses of genistein (10(-5) mol/L, 10(-6) mol/L and 10(-7) mol/L). The proliferation and DNA and collagen synthesis of osteoblasts were assayed by MTT method and 3H-TdR and 3H-proline incorporation. The activity of ALP were measured by ALP assay kit.</p><p><b>RESULTS</b>Genistein significantly increased osteoblast 3H-TdR and 3H-proline incorporation and MTT, 10(-6) mol/L genistein increased ALP activity.</p><p><b>CONCLUSION</b>Genistein increased osteoblast DNA and collagen synthesis in neonatal rat calvaria cultures, and promoted osteoblast proliferation and differentiation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Pharmacology
/
DNA
/
Cell Differentiation
/
Cells, Cultured
/
Collagen
/
Rats, Wistar
/
Genistein
/
Cell Biology
/
Cell Proliferation
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2003
Type:
Article
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