Effects of fluoride on ionized calcium and calcium channel in osteoblast-like cell in vitro / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To study the effects of fluoride (F-) on ionized calcium ([Ca2+]i) and calcium channel in osteoblast-like cell (OBLs).</p><p><b>METHODS</b>Investigating [Ca2+]i was by fluorospectrophotometry and recording the calcium currents of OBLs was using whole patch-clamp technique.</p><p><b>RESULTS</b>The addition of fluoride to the medium made a rapid and significant increase in free [Ca2+]i, especially in 100 ng F- /ml group. There were significances between control group and 50, 100 ng F- /ml groups. And 25 ng/ml F- could stimulate OBLs calcium channel open. Compared with control group, fluoride could significantly increase the amplitudes of calcium currents (P < 0.01), furthermore the activation was in dose-dependent manner.</p><p><b>CONCLUSION</b>Fluoride can make calcium channel open and then increase the concentrations of [Ca2+]i.</p>