Hyperglycemia inhibited taurine transport and taurine transporter gene expression in cultured rat cardiomyocytes / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 132-136, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-339660
ABSTRACT
<p><b>AIM</b>To investigate the alterations of taurine transport, and taurine transporter (TAUT) mRNA by hyperglycemia in cultured rat cardiomyocytes.</p><p><b>METHODS</b>3H-taurine measured the amount of taurine uptake. TAUT mRNA consents were measured using quantitative RT-PCR.</p><p><b>RESULTS</b>The cellular uptake amounts of taurine in seven groups increased with incubation time, and near to be saturated after 5 min. The uptake amount of 10, 20, and 30 mmol/L glucose groups was obviously lower than that of the control group (P < 0.05 or P < 0.01). In 30 mmmol/L glucose, taurine release obviously was decreased, as compared with that of the control. Exposure of cells to 10, 20, and 30 mmmol/L glucose decreased taurine uptake in a concentration-dependent fashion. Exposure to hyperglycemia did not affect the Km of the TAUT, but the apparent Vmax were significantly decreased (P < 0.05). In 20 and 30 mmmol/L groups, TAUT mRNA contents of myocardial cells were significantly reduced, as compared with the control group (P < 0.05).</p><p><b>CONCLUSION</b>The data suggests that there are dysfunction of taurine uptake and downregulation of TAUT gene expression by glucose in cultured rat cardiomyocytes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Membrane Transport Proteins
/
Pharmacology
/
Taurine
/
RNA, Messenger
/
Membrane Glycoproteins
/
Cells, Cultured
/
Rats, Sprague-Dawley
/
Myocytes, Cardiac
/
Genetics
/
Glucose
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2003
Type:
Article
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