Effect of 17beta-estradiol on phosphorylcholine cytidylyltransferase activity from cultured rat lung explants / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 57-59, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-339680
ABSTRACT
<p><b>AIM</b>To investigate the influence and mechanisms of 17beta-estradiol on the CTP phosphorylcholine cytidylyltransferase (CCT) activity from cultured lung explants without serum.</p><p><b>METHODS</b>We detected the amount of [M-14C] choline incorporation into phosphatidylcholine so as to reflect CCT activity by liquid scintillation.</p><p><b>RESULTS</b>(1) 17beta-estradiol increased the CCT activity in dose-dependence and time-dependence. (2) Both the protein kinase C inhibitor H-7 and calmodulin antagonist W-7 abolished the stimulatory effect of 17beta-estradiol (3 x 10(-6) mol/L) on the CCT activity.</p><p><b>CONCLUSION</b>17beta-estradiol can increase CCT activity in cultured lung explants, its mechanism is related to protein kinase C and calmodulin.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
In Vitro Techniques
/
Protein Kinase C
/
Calmodulin
/
Culture Media, Serum-Free
/
Rats, Wistar
/
Choline-Phosphate Cytidylyltransferase
/
Estradiol
/
Lung
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2003
Type:
Article
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