Measurement of nitrate and nitrite concentrations in biological fluids using high performance liquid chromatography / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 394-396, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-339701
ABSTRACT
<p><b>AIM AND METHODS</b>New method to analyse nitrate and nitrite concentrations in saliva, serum and urine was developed using high performance liquid chromatography.</p><p><b>RESULTS</b>The whole isolation process was completed in less than 7 minutes, the determination linearity of nitrate and nitrite were 0.7 ng-100 ng and 5 ng-100 ng, respectively. MINIMUM Detectable limits of nitrate and nitrite were 0.3 ng and 2 ng, respectively. Nitrate recovery ratio was 99%-102% and nitrite recovery ratio was 99%-104%. The RSD of nitrate and nitrite was 0.8% and 1.7%, respectively.</p><p><b>CONCLUSION</b>In comparison with other methods available, the present method seems to be simpler, more sensitive and specific.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Body Fluids
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Methods
/
Nitrates
/
Nitrites
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2002
Type:
Article
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