Mechanisms of protection effect of bcl-2 gene transfection on heat-stressed cardiomyocytes / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 347-349, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-339718
ABSTRACT
<p><b>AIM</b>To study the mechanisms of protection of bcl-2 gene transfection against heat-stressed cardiomyocytes.</p><p><b>METHODS</b>Cardiomyocytes were isolated and cultured. bcl-2 was transfected into cardiomyocytes with Lipofectamine transfection methods. The cardiomyocytes were stressed by heat. The change of H+ -ATPase synthesis activity of cardiomyocytes mitochondria caused by bcl-2 transfection was measured by chemical radiation method. The changes of Caspase 3 activity of cardiomyocytes caused by bcl-2 transfection was measured by fluorometric analysis.</p><p><b>RESULTS</b>bcl-2 transfection could increase the H+ -ATPase synthesis activity of cardiomyocytes mitochondria under heat stress at 41 degrees C and 43 degrees C and could decrease the Caspase 3 activity of cardiomyocytes under heat stress at 41 degrees C and 43 degrees C.</p><p><b>CONCLUSION</b>The protection effect of bcl-2 transfection on heat-stressed cardiomyocytes may be associated with preserved H+ ATPase synthesis activity of cardiomyocytes mitochondria and the activity of Caspase 3 of cardiomyocytes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Cells, Cultured
/
Proton-Translocating ATPases
/
Heat-Shock Response
/
Genes, bcl-2
/
Cell Biology
/
Myocytes, Cardiac
/
Caspase 3
/
Genetics
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2002
Type:
Article
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