Expression of periodontal ligament-associated protein-1 in normal periodontal tissues and cells in rat / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To examine the expression of periodontal ligament-associated protein-1 (PLAP-1) in the periodontal tissues and periodontal ligament cells (PDLC).</p><p><b>METHODS</b>The PLAP-1 expression in normal periodontal tissue was examined by immunohistochemistry. The protein expression and mRNA transcription of PLAP-1 in PDLC were investigated by immunocytochemistry and reverse transcription-polymerase chain reaction.</p><p><b>RESULTS</b>PLAP-1 was expressed in periodontium but not in cementum, alveolar bone and gingival tissues. PLAP-1 expression was observed in cell plasma, but not in nuclei. There was a 350 bp electrophoresis band representing PLAP-1 mRNA.</p><p><b>CONCLUSIONS</b>PLAP-1 may play a role in physiology of periodontal tissues and cells in normal adult rats.</p>