Relationship between Sp3 and the transcriptional regulation of enamelin gene / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 165-167, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-339782
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of Sp3 in the transcriptional regulation of enamelin gene.</p><p><b>METHODS</b>By bioinformatic analysis, a putative responsive element for Sp3 was identified. Electrophoretic mobility shift assay was used to examine the interaction between Sp3 and enamelin. 5'-flanking regulatory region of enamelin was cloned and ligated into pGL3-basic luciferase vector. Sp3 and the Enam-luc were cotransfected into mouse ameloblast-like cell line, and the activity of luciferase was examined.</p><p><b>RESULTS</b>The results showed that Sp3 could not directly bind to the enamelin regulation region and activate enamelin transcription.</p><p><b>CONCLUSIONS</b>Sp3 might not be involved in transcriptional regulation of enamelin gene via an indirect interaction.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transcription, Genetic
/
Transfection
/
Transcriptional Activation
/
Cell Line
/
Gene Expression Regulation
/
Promoter Regions, Genetic
/
Rats, Sprague-Dawley
/
Genes, Reporter
/
Cell Biology
/
Electrophoretic Mobility Shift Assay
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2011
Type:
Article
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