The use of dental papillae cells and millipore filter for bioengineered dentin / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 678-683, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-339825
ABSTRACT
<p><b>OBJECTIVE</b>To observe the ability of SD rat dental papillae cells forming dentin-like structure induced by millipore filter combined with transforming growth factor-β(1) (TGF-β(1)).</p><p><b>METHODS</b>The first passage SD rat dental papillae cells were enzymatically dissociated and centrifuged to obtain a cell mass. The cell mass was seeded on the millipore filter combined with TGF-β(1). The complex was incubated for 6 d in vitro or transplanted under the renal capsule for 2 weeks. Then the differentiation of dental papillae cells on the filter and the formation of mineral tissue on the implant were analyzed.</p><p><b>RESULTS</b>A layer of polarized columnar cells were observed along the surface of the millipore filter, with cell processes extending into the porous media. Dentin sialoprotein (DSP) and dentin matrix protein-1 (DMP-1) were positive in these cells. After 2 weeks, tubular dentin matrix was deposited on the surface of the aligned cells. Scanning electron microscopy showed that the thickness of newly formed tubular dentin was consistent. DSP and DMP-1 were expressed in columnar cells, tubular matrix and the dental papillae cells adjacent to the filter.</p><p><b>CONCLUSIONS</b>The millipore filter combined with TGF-β(1) could effectively recruit progenitors onto its surface and induce odontoblast differentiation, secrete matrix in a homogenous manner, leading to dentinogenesis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phosphoproteins
/
Sialoglycoproteins
/
Cell Differentiation
/
Cells, Cultured
/
Extracellular Matrix Proteins
/
Transforming Growth Factor beta
/
Cell Biology
/
Tissue Engineering
/
Dental Papilla
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2010
Type:
Article
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