Cloning and sequence analysis of the DHBV genome of the brown ducks in Guilin region and establishment of the quantitative method for detecting DHBV / 病毒学报
Chinese Journal of Virology
;
(6): 180-184, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-339955
ABSTRACT
Brown ducks carrying DHBV were widely used as hepatitis B animal model in the research of the activity and toxicity of anti-HBV dugs. Studies showed that the ratio of DHBV carriers in the brown ducks in Guilin region was relatively high. Nevertheless, the characters of the DHBV genome of Guilin brown duck remain unknown. Here we report the cloning of the genome of Guilin brown duck DHBV and the sequence analysis of the genome. The full length of the DHBV genome of Guilin brown duck was 3 027bp. Analysis using ORF finder found that there was an ORF for an unknown peptide other than S-ORF, PORF and C-ORF in the genome of the DHBV. Vector NTI 8. 0 analysis revealed that the unknown peptide contained a motif which binded to HLA * 0201. Aligning with the DHBV sequences from different countries and regions indicated that there were no obvious differences of regional distribution among the sequences. A fluorescence quantitative PCR for detecting DHBV was establishment based on the recombinant plasmid pGEM-DHBV-S constructed. This study laid the groundwork for using Guilin brown duck as a hepatitis B animal model.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Poultry Diseases
/
Virology
/
Molecular Sequence Data
/
Base Sequence
/
Hepatitis B Virus, Duck
/
China
/
Polymerase Chain Reaction
/
Epidemiology
/
Classification
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2013
Type:
Article
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