Development of SYBR Green I real-time RT-PCR for the detection of Ebola virus / 病毒学报
Chinese Journal of Virology
;
(6): 567-571, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-340004
ABSTRACT
In order to establish a rapid and accurate method for the detection of Ebola virus (EBOV), the primers used in SYBR Green I real-time RT-PCR were designed based on the EBOV NP gene sequences published in GenBank. The SYBR Green I real-time RT-PCR was established and optimized for the detection of EBOV. The EBOV RNA that was transcribed in vitro was used as a template. The sensitivity of this method was found to reach 1.0 x 10(2) copies/microL and the detection range was 10(2) - 10(10). No cross reaction with RNA samples from Marburg virus, Dengue virus, Xinjiang hemorrhagic fever virus, Japanese encephalitis virus, Influenza virus (H1N1 and H3N2) and Porcine reproductive and respiratory syndrome virus E genomic RNA was found. The method would be useful for the detection and monitoring of EBOV in China.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organic Chemicals
/
Virology
/
Chemistry
/
DNA Primers
/
Hemorrhagic Fever, Ebola
/
Reverse Transcriptase Polymerase Chain Reaction
/
Ebolavirus
/
Genetics
/
Methods
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2012
Type:
Article
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